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目的:研究蒙药色玛-3汤对大鼠慢性肾衰模型的治疗作用,探讨其作用机制。方法:将50只Wister大鼠随机分为5组:空白对照组、病理模型组、色玛-3汤高剂量治疗组(20g·kg-1·d-1)、色玛-3汤中剂量治疗组(10g·kg-1·d-1)、色玛-3汤低剂量治疗组(5g·kg-1·d-1),每组10只。以腺嘌呤200mg·kg-1·d-1灌胃建立CRF模型,造模同时各治疗组灌胃给予章古-3汤。连续灌胃24d后,检测生化学指标尿素氮(BUN)、血清肌酐(Scr);观察肾组织病理改变;用免疫组化方法检测肾组织中TNF-α的表达和分布。结果:经色玛-3汤治疗的各组大鼠与同期病理模型组比较,肾功能(BUN,Scr清除水平)有明显的改善(P<0.05),病理改变较轻。治疗组TNF-α表达水平显著低于同期病理模型组(P<0.05)。结论:蒙药色玛-3汤大鼠CRF模型的肾功能有一定保护作用,其作用机制可能与抑制TNF-α的表达有关。
Objective: To study the therapeutic effect of Mongolian medicine Sema-3 on chronic renal failure model in rats and its mechanism of action. Methods: Fifty Wister rats were randomly divided into five groups: blank control group, pathological model group, high-dose Sema-3 soup treatment group (20g · kg-1 · d-1) The treatment group (10g · kg-1 · d-1), Sema-3 low-dose treatment group (5g · kg-1 · d-1), 10 in each group. CRF model was established by gavage with adenine 200mg · kg-1 · d-1, and the model group was given GZ-3 soup by intragastric administration. After 24 days of continuous gavage, biochemical indicators of blood urea nitrogen (BUN) and serum creatinine (Scr) were detected. The pathological changes of renal tissues were observed. The expression and distribution of TNF-α in renal tissues were detected by immunohistochemistry. Results: Compared with the same pathological model group, the renal function (BUN and Scr clearance) of rats in each group treated by Sema-3 was significantly improved (P <0.05), and the pathological changes were mild. The level of TNF-α in the treatment group was significantly lower than that in the same period (P <0.05). CONCLUSION: The renal function of CRF model in Mongolian medicine Sema-3 treated rats may have a protective effect, and its mechanism may be related to the inhibition of TNF-α expression.