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目的:观察情志刺激对动脉粥样硬化(AS)形成过程的影响,阐述硫化氢(H2 S)在动脉粥样硬化病变中的作用.方法:24只SD大鼠随机分为AS组、气滞血瘀型AS组和正常对照组,AS组给予特殊饲料,气滞血瘀型AS组给予特殊饲料+情志刺激,正常对照组给予普通饲料.造模期间,分别在第4、第8、第12周测定大鼠表征评分、H2 S含量、凝血四项、组织血流量、血脂指标.结果:气滞血瘀型AS组与AS组相比,第4周开始大鼠表征出现活动量明显减少、反应迟钝、被毛无光泽、舌质紫黯等现象(P<0.01);血脂明显升高,并随时间的增长而升高(P<0.05或P<0.01);第8周开始活化部分凝血活酶时间(APTT)、纤维蛋白原(FIB)明显升高(P<0.05);第8周开始表皮、肝脏、肾脏组织血流量均明显减少;第12周时H2S的含量明显升高(P<0.01).结论:情志刺激促进了AS模型的形成,同时气体分子H2S体系在气滞血瘀型AS动物模型中也发挥了调节作用.“,”Objective:To observe the effect of emotional stimulation on the formation process of atherosclerosis ( AS) ,and explain the role of hydrogen sulfide ( H2 S) in atherosclerotic lesions. Methods:Twenty-four Sprague-Dawley rats were randomly divided into AS group, qi-stagnation and blood-stasis AS group and the control group. The AS group was fed with special diets, the qi-stagnation and blood-stasis AS group was fed with special diets and emotional stimulation, and the control group was fed with normal diets. During the experiment, the indicators including the characterization score, H2 S content, four items of the natural bleeding and blood coagula-tion, tissue blood flow and blood lipid were respectively detected in the 4 th, 8 th and 12 th week. Results:Compared with those in the AS group, since the 4 th week, the rats in qi-stagnation and blood-stasis AS group were with significantly decreased activity, slow re-sponse, lackluster fur and dark purple tongue (P<0. 01);the level of plasma lipid increased significantly, and increased further with time extension(P<0. 05 or P<0. 01);since the 8 th week, APTT and FIB changed significantly (P<0. 05),the blood flow to skin, liver and kidney decreased significantly (P<0. 05);the content of H2S was significantly higher in the 12 th week (P<0. 01). Con-clusion:Emotional stimulation promotes the formation of AS model,and the gas molecule system of H2 S plays a regulatory role in the qi-stagnation and blood-stasis AS animal model.