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目的探讨儿童氟斑牙与维生素D受体(VDR)FokⅠ基因多态性的关系。方法选取河南省开封、通许2县8～12岁儿童高氟区143人和对照区94人为对象,按照氟斑牙患病情况分为高氟区患者组,高氟区非患者组及对照组;采用氟离子选择电极法检测尿氟含量,利用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析雌激素受体(VDR)FokⅠ基因多态性。结果高氟区儿童氟斑牙患病率为51.7%,对照区未检出氟斑牙患者;高氟区VDR FokⅠ基因型分布频率氟斑牙患者为FF 32.4%(24/74),Ff 45.9%(34/74),ff 21.6%(16/74);非患者为FF40.6%(28/69),Ff 36.2%(25/69),ff 23.2%(16/69),对照区儿童为FF 31.9%(30/94),Ff 50.0%(47/94),ff 18.1%(17/94);3组儿童基因型频率分布差异无统计学意义(P>0.05);VDR FokⅠ基因型在高氟区不同性别儿童中分布相同,差异无统计学意义(P>0.05);VDR FokⅠ基因型在尿氟超标的氟斑牙患者和非患者中分布相同,差异无统计学意义(P>0.05)。结论高氟区儿童氟斑牙发生与VDR FokⅠ基因型无明显关系。
Objective To investigate the relationship between Fluorokinase (Fok Ⅰ) gene polymorphism and vitamin D receptor (VDR) in children. Methods A total of 143 children in 8 ~ 12 years old children aged 8 ~ 12 in Tonghua County of Henan Province and 94 persons in control group were enrolled in this study. According to the prevalence of dental fluorosis, they were divided into high fluoride group, non-high fluoride group and control group ; Fluoride ion-selective electrode method was used to detect urinary fluoride content, and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the FokⅠ gene polymorphism of estrogen receptor (VDR). Results The prevalence of dental fluorosis was 51.7% in children with high fluoride area and no dental fluorosis was found in the control area. The frequency of VDR FokⅠ genotype in high fluoride area was FF 32.4% (24/74) and Ff 45.9 % (34/74) and ff 21.6% (16/74) respectively. The non-patients were FF 40.6% (28/69), Ff 36.2% (25/69) and ff 23.2% (16/69) Ff 50.0% (47/94) and ff 18.1% (17/94) respectively. There was no significant difference in genotype frequencies among the three groups (P> 0.05) The distribution of VDR FokⅠ genotypes was the same among fluorosis patients with fluorosis and non-patients, the difference was not statistically significant (P> 0.05). Conclusions The incidence of dental fluorosis in children with high fluoride is not related to VDR Fok Ⅰ genotype.