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目的：了解外源性钙离子对放烧复合伤心肌线粒呼吸功能的影响。方法：Ｗｉｓｔａｒ大鼠分为正常对照组，６Ｇｙ照射复合３０％ＴＢＳＡⅢ度烧伤组、放烧复合伤预防用药组和放烧复合伤治疗用药组。于伤后１、１２、２４和４８ｈ测定不同浓度钙对各组线粒体呼吸功能的影响。结果：低浓度外源性钙（１０μｍｏｌ／Ｌ）对正常对照组心肌线粒体呼吸有刺激作用，但对放烧复合伤组、放烧复合伤预防用药组和治疗用药组线粒体呼吸无显著刺激作用；高浓度外源性钙（５０μｍｏｌ／Ｌ）对正常组、放烧复合伤预防用药组和治疗用药组心肌线粒体呼吸功能有显著抑制作用，但使复合伤组心肌线粒体呼吸完全脱偶联。１８７．５μｍｏｌ／Ｌ维拉帕米可显著减轻５０μｍｏｌ／Ｌ钙离子对呼吸的抑制。结论：放烧复合伤心肌线粒体对外源性钙的耐受性降低，维拉帕米预防或治疗用药均能提高复合伤后线粒体对外源性钙的耐受性。
Objective: To investigate the effect of exogenous Ca2 + on respiratory function of mitochondria induced by radiation and burn. Methods: Wistar rats were divided into normal control group, 6Gy irradiation combined with 30% TBSA Ⅲ degree burn group, burn combined injury prevention group and burn combined burn injury treatment group. The effects of different concentrations of calcium on mitochondrial respiratory function in each group were determined at 1, 12, 24 and 48 h after injury. Results: Low concentration of exogenous calcium (10μmol / L) stimulated mitochondrial respiration in normal control group, but there was no significant stimulation on mitochondrial respiration in radiotherapy and burn combined injury prevention group and treatment group. High concentration of exogenous calcium (50μmol / L) had significant inhibitory effect on myocardial mitochondrial respiratory function in normal group, combination of prophylaxis with burn and burn therapy, and treatment group, but completely uncoupled myocardial mitochondrial respiration in combined injury group. 187.5μmol / L verapamil can significantly reduce the respiratory inhibition of 50μmol / L calcium ion. CONCLUSION: The mitochondria exposed to burn and burn myocardial mitochondria are less resistant to exogenous calcium. Verapamil prophylaxis or treatment can increase the mitochondrial tolerance to exogenous calcium after the composite injury.