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目的为保证实验室测试结果的准确性和可靠性,采用液相色谱-串联质谱法对肠衣中12种磺胺类药物残留量测定的不确定度进行评估。方法按照《测量不确定度评定与表示》的要求,对GB/T 20759-2006《畜禽肉中十六种磺胺类药物残留量的测定液相色谱-串联质谱法》进行肠衣中12种磺胺类药物测定的不确定度分析。结果建立了相应的数学模型,对测量结果的不确定度来源,如标准物质、标准溶液配制、样品称量、样品定容、样品回收率及结果的重复性等进行了量化分析。结论标准溶液的配制及稀释、样品回收率对样品的合成不确定度贡献较大。
Objective To ensure the accuracy and reliability of laboratory test results, the uncertainty of determination of 12 sulfonamides residues in enteric coating was evaluated by liquid chromatography-tandem mass spectrometry. Methods According to the requirements of “Evaluation and Expression of Uncertainty in Measurement”, 12 kinds of sulfonamides in enteric coating were determined on GB / T 20759-2006 “Determination of 16 kinds of sulfonamides in livestock and poultry meat by liquid chromatography-tandem mass spectrometry” Uncertainty Analysis of Drug Determination. Results The corresponding mathematical model was established to quantitatively analyze the sources of uncertainty of the measurement results, such as standard substance, standard solution preparation, sample weight, sample volume, sample recovery rate and repeatability of results. Conclusion Standard solution preparation and dilution, the sample recovery rate of the synthesis of the greater contribution to the uncertainty of the sample.