Background: RNA binding proteins (RBPs) play essential roles in the regulation of RNA metabolism.Recent studies have disclosed that RBPs achieve their functions via binding to their targets in a position-dependent patt on RNAs.However,few studies have systematically addressed the associations between the RBP’s functions and their positional binding preferences.Methods: Here,we present large-scale analyses on the functional targets of human RBPs by integrating the enhanced cross-linking and immunoprecipitation followed by sequencing (eCLIP-seq) datasets and the shRNA knockdown followed by RNA-seq datasets that are deposited in the integrated ENCyclopedia of DNA Elements in the human genome (ENCODE) data portal.Results: We found that (1) binding to the translation termination site and the 3’untranslated region is important to most human RBPs in the RNA decay regulation;(2) RBPs’ binding and regulation follow a cell-type specific patt.Conclusions: These analysis results show the strong relationship between the binding position and the functions of RBPs,which provides novel insights into the RBPs’ regulation mechanisms.