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目的:探讨青岛地区汉族人群阿司匹林抵抗与血小板膜糖蛋白GPⅢaPLA、Ⅰa807C/T基因多态性之间的关系。方法:筛选150例动脉粥样硬化患者服用阿司匹林(100mg/d)至少14d以上,根据血小板聚集功能测定将其分为阿司匹林抵抗(AR)组、阿司匹林半抵抗(ASR)组,阿司匹林敏感(AS)组。用PCR-RFLP法确定各组GPⅢaPLA、GPⅠa 807C/T基因型。结果:仅于ASR组检出1例PLA1/A2基因型,其余均为PLA1/A1基因型,未发现PLA2/A2基因型,差异无统计学意义(P>0.005);GPⅠa807C/T基因位点AR组、ASR组的T等位基因频率均显著高于AS组,有统计学意义(P<0.005)。结论:GPⅢaPLA2基因可能不是阿司匹林抵抗的遗传危险因素。而GPⅠa 807C/T基因位点的T等位基因与阿司匹林抵抗的发生相关联,可能是阿司匹林抵抗遗传易感因素。
Objective: To investigate the relationship between aspirin resistance and GPⅢaPLA and Ⅰa807C / T polymorphisms of platelet membrane glycoprotein in Han nationality in Qingdao area. Methods: A total of 150 patients with atherosclerosis were selected for aspirin (100mg / d) for at least 14 days. According to their platelet aggregation function, they were divided into five groups: aspirin resistance group, aspirin half-resistance group, aspirin- group. GPⅢaPLA and GPⅠa 807C / T genotypes were determined by PCR-RFLP. Results: PLA1 / A2 genotype was found in only one case of ASR, and PLA1 / A1 genotype was not found in PLA1 / A1 genotype. The difference was not statistically significant (P> 0.005). The GPⅠa807C / T gene locus The frequencies of T allele in AR group and ASR group were significantly higher than those in AS group (P <0.005). Conclusion: GPⅢaPLA2 gene may not be a genetic risk factor for aspirin resistance. The T allele of GPⅠa 807C / T gene locus was associated with the occurrence of aspirin resistance, which may be the predisposing factor of aspirin resistance.