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目的观察丙泊酚对大鼠脑缺血再灌注后脑水肿及表皮生长因子受体(EGFR)和细胞外信号调节蛋白激酶(ERK1/2)磷酸化的影响。方法将60只SD大鼠按随机数字表法分为6组:假手术组(Sham组)、大脑中动脉栓塞(MCAO)2h模型组(I组)、MCAO 2h再灌注24h模型组(I/R组)、丙泊酚预处理假手术组(Pro组)、丙泊酚预处理MCAO 2h模型组(Pro+I组)及丙泊酚预处理MCAO 2h再灌注24h模型组(Pro+I/R组),每组10只。将各组制模成功后大鼠断头取脑组织,采用干重法测定缺血区脑组织含水量,采用Western Blot检测EGFR和ERK1/2磷酸化水平。结果 I/R组缺血区脑组织含水量较I组和Sham组显著增加(P<0.05),EGFR和ERK1/2磷酸化水平较I组和Sham组显著升高(P<0.05);Pro+I/R组脑组织含水量较I/R组显著降低(P<0.05),EGFR和ERK1/2磷酸化水平较I/R组显著下降(P<0.05)。Sham组、I组、Pro组、Pro+I组及Pro+I/R组的缺血区脑组织含水量、EGFR和ERK1/2磷酸化水平比较差异均无统计学意义(P>0.05)。结论丙泊酚能有效通过抑制EGFR和ERK1/2磷酸化降低脑缺血再灌注大鼠脑水肿程度。
Objective To observe the effect of propofol on brain edema and the phosphorylation of epidermal growth factor receptor (EGFR) and extracellular signal-regulated protein kinase (ERK1 / 2) after cerebral ischemia-reperfusion in rats. Methods Sixty Sprague-Dawley rats were randomly divided into 6 groups: sham group (Sham group), middle cerebral artery occlusion group (MCAO) for 2 hours, MCAO group for 2 hours and reperfusion group (I / R group, propofol preconditioning sham operation group (Pro group), propofol preconditioning MCAO 2h model group (Pro + I group) and propofol preconditioning MCAO 2h reperfusion 24h model group (Pro + I / R group), 10 in each group. The rats were sacrificed after decapitation in each group. The brain water content in the ischemic area was determined by dry weight method. The phosphorylation levels of EGFR and ERK1 / 2 were detected by Western Blot. Results The brain water content in ischemic zone in I / R group was significantly higher than that in I group and Sham group (P <0.05). The phosphorylation of EGFR and ERK1 / 2 in I / R group was significantly higher than that in I group and Sham group (P <0.05) The brain water content of + I / R group was significantly lower than that of I / R group (P <0.05), and the phosphorylation of EGFR and ERK1 / 2 was significantly lower than that of I / R group (P <0.05). Sham group, I group, Pro group, Pro + I group and Pro + I / R group had no significant difference in brain water content, EGFR and ERK1 / 2 phosphorylation (P> 0.05). Conclusion Propofol can effectively reduce brain edema in rats with cerebral ischemia / reperfusion by inhibiting EGFR and ERK1 / 2 phosphorylation.