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Objective To observe the effects of glutamate on sodium channel in acutely dissociated hippocampal CAI pyramidal neurons of rats. Methods Voltage-dependent sodium currents ( Ina ) in acutely dissociated hippocampal CAI pyramidal neurons of neonate rats were recorded by whole-cell patchclamp of the brain slice technique when a series of doses of glutamate (100- 1000 μmol/L ) were applied. Results Different concentrations of glutamate could inhibit Ina, and higher concentration of glutamate affected greater inhibition. In same concentration on Ina at different times, longer time glutamate affected greater inhibition. And glutamate could significantly shift activation curve of Ina to the right and make inactivation curve of Ina negative drift. Conclusion Glutamate can inhibit voltage-dependent sodium channel. Its blockage on Ina has voltage-dependent, time-dependent, and dose-dependent characteristics.