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采用405 nm发光二极管(LED)作为激发光源,选用孟加拉红(RB)作为光敏剂,可对早期口腔肿瘤进行诊断。正常的口腔黏膜不会被孟加拉红染色,而当口腔产生癌前病变或者生成肿瘤时,孟加拉红就会将病变部位染色。通过光谱测量可知,被染色病变部位的特征荧光光谱波长范围为570~600 nm,而正常口腔组织的特征荧光光谱中心波长约在480 nm。当癌前病变发展成恶性肿瘤之后,其特征荧光光谱发生改变,在630 nm和690 nm处各有一个典型的卟啉峰。以此为依据并结合光致荧光技术,采用荧光比例法,可以对口腔癌前病变进行诊断,灵敏度和特异性分别可达到95%和92.5%。这种无创、快速、早期的诊断方法,可以显著提高口腔癌患者的存活率。
405 nm light-emitting diode (LED) is used as the excitation light source, and bengal red (RB) is selected as the photosensitizer to diagnose early oral tumors. Normal oral mucosa will not be Bengal red staining, and when the oral precancerous lesions or tumor formation, Bengal red will stain the lesion. By spectral measurement, we can see that the characteristic fluorescence spectrum wavelength range of the stained lesion is 570-600 nm, while the characteristic fluorescence spectrum center wavelength of normal oral tissue is about 480 nm. When precancerous lesions develop into malignant tumors, their characteristic fluorescence spectra change, with a typical porphyrin peak at 630 nm and 690 nm. Based on this, combined with photofluorescence, the fluorescent ratio method can be used to diagnose oral precancerous lesions with sensitivity and specificity of 95% and 92.5% respectively. This non-invasive, rapid, early diagnosis can significantly improve the survival rate of oral cancer patients.