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目的探讨膜表面核仁素(nucleiolin,NCL)在表皮生长因子受体(epidermal growth factor receptor,EGFR)信号启动中的作用。方法采用免疫组化法检测NCL及EGFR在甲状腺乳头状癌组织中的表达;Western blot法检测甲状腺乳头状癌细胞TPC-1中磷酸化EGFR(phosphorylation EGFR,p-EGFR)的表达水平;Transwell小室试验检测TPC-1细胞的迁移能力。结果免疫组化染色结果显示,甲状腺乳头状癌组织中NCL及EGFR的表达阳性率分别为100%(56/56)和80.4%(45/56);NCL及EGFR的表达与淋巴结转移有关(P<0.05),且NCL的表达与EGFR的表达呈正相关关系(r=0.635,P<0.01)。Western blot法检测结果显示,拮抗甲状腺乳头状癌TPC-1细胞的NCL或EGFR后,其p-EGFR表达水平均明显下调(P<0.01)。Transwell小室试验发现,拮抗NCL和EGFR后,可明显减少TPC-1细胞的穿膜细胞数(P<0.01)。结论膜表面NCL可能是EGFR受体信号启动的必要成分,可能通过EGFR参与肿瘤的生长与转移。以NCL为靶点将开拓肿瘤新的治疗领域。
Objective To investigate the role of nuclei (NCL) on the activation of epidermal growth factor receptor (EGFR) signaling. Methods The expression of NCL and EGFR in papillary thyroid carcinoma tissues was detected by immunohistochemistry. The expression of phosphorylated EGFR (p-EGFR) in papillary thyroid carcinoma TPC-1 cells was detected by Western blot. TPC-1 cells were tested for their ability to migrate. Results The positive rates of NCL and EGFR in papillary thyroid carcinoma were 100% (56/56) and 80.4% (45/56), respectively. The expressions of NCL and EGFR were correlated with lymph node metastasis <0.05). There was a positive correlation between NCL expression and EGFR expression (r = 0.635, P <0.01). The results of Western blot showed that the expression of p-EGFR in TPC-1 cells was significantly down-regulated after NCL or EGFR treatment (P <0.01). Transwell chamber assay found that antagonizing NCL and EGFR significantly decreased the number of transmembrane cells in TPC-1 cells (P <0.01). Conclusion NCL on the membrane surface may be an essential component of the activation of EGFR receptor signaling and may be involved in tumor growth and metastasis through EGFR. The NCL as a target will open up new areas of cancer treatment.