论文部分内容阅读
目的 :构建凋亡信号受体Fas/APO - 1基因真核表达质粒 ,为进一步研究Fas/APO - 1在肿瘤细胞凋亡中的作用提供物质基础。方法 :将质粒pBluscript-FascDNA酶切后 ,回收DNA片段并提纯 ;用连接酶连接到真核表达载体pBK CMV内 ,克隆出重组基因载体pBK FascDNA。结果 :原始质粒pBluscript-FascDNA及重组质粒pBK -FascDNA酶切后 ,凝胶电泳鉴定均含有大小正确的Fas/APO - 1碱基片段。结论 :本实验成功地构建了Fas/APO - 1基因的真核表达质粒。
OBJECTIVE: To construct eukaryotic expression plasmid of Fas / APO - 1 signal transducers and to provide the material basis for further study on the role of Fas / APO - 1 in tumor cell apoptosis. METHODS: The plasmid pBluscript-Fasc DNA was digested and the DNA fragments were recovered and purified. The ligated enzyme was ligated into the eukaryotic expression vector pBK CMV, and the recombinant gene vector pBK Fasc DNA was cloned. Results: After digestion with pBluscript-Fasc DNA and pBK-Fasc DNA, the correct size of Fas / APO - 1 was detected by gel electrophoresis. Conclusion: The eukaryotic expression plasmid of Fas / APO - 1 gene was successfully constructed in this experiment.