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目的 利用基因表达谱芯片对NB4细胞在硫化砷作用前后基因表达的差异性进行比较。方法 用Cy3和Cy5两种不同的荧光染料通过逆转录反应将硫化砷处理前后的NB4细胞mRNA分别标记成两种探针 ,并与载有一组靶基因的基因表达谱芯片进行杂交 ,通过扫描、计算机软件分析 ,寻找经硫化砷作用后表达有差异的基因。结果 筛选出包括与细胞凋亡、DNA合成修复、蛋白翻译、细胞周期等相关的硫化砷作用前后表达有差异的基因共 34条 ,其中 6条表达上调 ,2 8条表达下调。结论 ABC5 0、PNAS 2、周期素G2 可能参与硫化砷诱导NB4细胞凋亡的发生机制
Objective To compare the gene expression differences of NB4 cells before and after the arsenic sulfide treatment by using gene expression microarray. Methods The mRNA of NB4 cells before and after arsenic sulfide treatment were labeled with two kinds of probes by Cy3 and Cy5 fluorescent dyes respectively and then hybridized with a gene expression microarray carrying a set of target genes. Computer software analysis, looking for the role of arsenic sulfide expression differences after the gene. Results A total of 34 genes, including 6 genes were up-regulated and 28 genes were down-regulated, including the expression of arsenic sulfide before and after apoptosis related to apoptosis, DNA synthesis and repair, protein translation and cell cycle. Conclusions ABC5 0, PNAS 2 and cyclin G2 may be involved in the mechanism of apoptosis induced by As 3 S 3 in NB4 cells