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Dry heat treatment has been identifie as a method for disinfecting seed-borne pathogens in vegetable seeds. This study demonstrated that three seed-borne pathogens of cucumber(Cladosporium cucumerinum that causes scabs, Ascochyta citrullina that results in gummy stem blight,and Colletotrichum orbiculare that induces anthracnose) could be effectively eradicated from cucumber seeds by dry heat treatment. In vitro growth of these three pathogens was inhibited by dry heat treatment at 70 °C for 40 min. These pathogens were inactivated after exposing infected seeds to 70 °C dry heat for at least 90 min. Seed infection was significant y reduced by exposing the seeds to 70 °C dry heat for at least 40 min. Seed moisture content and germination were slightly reduced after 70 °C heat treatment for 40–120 min. Seed vigor remained at a high level after dry heat treatment at 70 °C for 90 min. In conclusion, 70 °C dry heat treatment for 90 min was determined to be the optimal method for eradication of C. cucumerinum, Didymella bryoniae, and C. orbiculare from cucumber seeds.
This study demonstrates that the three seed-borne pathogens of cucumber (Cladosporium cucumerinum that causes scabs, Ascochyta citrullina that results in gummy stem blight, and Colletotrichum orbiculare that induces anthracnose) could be effectively eradicated from cucumber seeds by dry heat treatment. In vitro growth of these three pathogens was inhibited by dry heat treatment at 70 ° C for 40 min. These pathogens were inactivated after exposing the infected seeds to 70 ° C dry heat for at least 90 min. Seed infection was significant y reduced by exposing the seeds to 70 ° C dry heat for at least 40 min. Seed moisture content and germination were slightly reduced after 70 ° C heat treatment for 40-120 min. remained at a high level after dry heat treatment at 70 ° C for 90 min. In conclusion, 70 ° C dry heat treatment for 90 min was determined to be the optimal method for erad ication of C. cucumerinum, Didymella bryoniae, and C. orbiculare from cucumber seeds.