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目的:研究银翘解毒口服液对流感病毒FM1感染的T,B细胞缺陷小鼠(SCID)血清TNF-α及TGF-β1含量的影响。方法:除正常对照组外,其余小鼠在感染流感病毒鼠肺适应株FM1后,分为模型组、阳性药组、银翘解毒口服液低、中、高剂量组,按组别分别灌胃,分别于感染后第1,3,5,7天采用双抗体夹心ABC-ELISA法动态观察血清血清TNF-α及TGF-β1含量的变化。结果:银翘解毒口服液治疗后,小鼠血清中TNF-α含量有所降低,中、高剂量组的调节作用明显于低剂量组;血清TGF-β1含量有所增加,中高剂量组作用显著。从感染时相上看,从第3天起,血清中TGF-β1含量开始升高,到第5天达最大值,第7天有所回落;TNF-α含量从第3天开始降低并持续到第7天。结论:银翘解毒口服液能够提高血清TGF-β1含量,降低TNF-α含量,维持免疫系统的平衡。
Objective: To study the effect of Yinqiao Jiedu oral liquid on the levels of serum TNF-α and TGF-β1 in T, B cell-deficient mice (SCID) infected with influenza virus FM1. Methods: Except the normal control group, the other mice were divided into model group, positive group and Yinqiao Jiedu oral low, medium and high dose groups after inoculation of FM1 lung adaptive strain FM1, The changes of serum TNF-αand TGF-β1 in serum were detected by double-antibody sandwich ABC-ELISA on days 1, 3, 5, and 7 respectively. Results: After treatment with Yinqiao Jiedu oral liquid, the serum TNF-αlevels decreased, while the medium and high dose groups had obvious effects on the regulation of low-dose group. The levels of serum TGF-β1 increased and the middle- and high-dose groups had significant effects . From the third phase, the content of TGF-β1 began to increase in the phase of infection, reached the maximum on the fifth day, and then decreased on the seventh day. The level of TNF-α decreased from the third day and continued By the seventh day. Conclusion: Yinqiao Jiedu oral liquid can increase the content of serum TGF-β1, decrease the content of TNF-α and maintain the balance of immune system.