论文部分内容阅读
目的 探讨抗心磷脂抗体 (ACL)阳性习惯性流产与人类白细胞抗原 DQ区域 (HLA DQ)等位基因多态性的关系。方法 采用聚合酶链反应 限制性片段长度多态性方法 ,对 30例ACL(+)习惯性流产患者进行HLA DQA1和HLA DQB1基因型的分析 ,并以 90例无不良妊娠史妇女为对照。结果 在DQB1等位基因表达频率中 ,DQB1 0 30 3等位基因表达频率在ACL(+)习惯性流产患者为33.3% ,比对照者明显增高 (P <0 .0 5 ) ;在其他DQB1等位基因表达频率上 ,以及DQA1各等位基因和DQA1 DQB1各单元型表达频率上 ,两者间差异均无显著性 (P >0 .0 5 )。结论 DQB1 0 30 3等位基因与ACL(+)习惯性流产相关 ,可能是ACL(+)习惯性流产的易感基因。
Objective To investigate the relationship between anticardiolipin antibody (ACL) positive habitual abortion and human leukocyte antigen DQ region (HLA DQ) allele polymorphism. Methods The genotypes of HLA DQA1 and HLA DQB1 in 30 patients with ACL (+) habitual abortion were analyzed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). 90 women without history of adverse pregnancy were used as controls. Results The frequencies of allele DQB1303 in DQB1 allele were 33.3% in patients with ACL (+) habitual abortion, which were significantly higher than those in controls (P <0.05). In other DQB1 et al There was no significant difference in the frequency of the expression of DQA1, DQA1 alleles and DQA1 DQB1 haplotype (P> 0.05). Conclusion DQB1 0303 allele is associated with ACL (+) habitual abortion and may be a susceptible gene for ACL (+) habitual abortion.