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目的 :探讨整合素α6、β4在人喉癌细胞Hep 2和AMC HN 8中的表达及其对肿瘤细胞体外黏附性状的影响及机制。方法 :应用流式细胞术检测Hep 2和AMC HN 8人喉癌细胞株整合素α6、β4的表达 ;采用整合素亚基α6、β4特异性单抗对喉癌细胞进行黏附抑制试验与快速体外浸润实验。结果 :Hep 2和AMC HN 8细胞株均表达较高的整合素α6、β4 ,其阳性细胞百分率分别为 (4 5 .73± 2 .31) %、(2 5 .76± 6 .6 3) %和 (4 5 .5 2± 15 .5 2 ) %、(84 .5 0± 16 .5 4) %。α6特异性单抗对Hep 2细胞株有明显黏附抑制作用 (P <0 .0 5 ) ,β4特异性单抗则无明显黏附抑制作用 (P>0 .0 5 ) ,抗α6加抗β4黏附抑制作用更为明显 (P <0 .0 1) ;抗α6或抗 β4对AMC HN 8细胞株均有明显黏附抑制作用 (均P <0 .0 5 ) ,抗α6加抗 β4其抑制作用更明显 (P <0 .0 1)。结论 :整合素α6、β4在喉癌细胞的黏附和浸润过程中起重要作用。利用α6、β4特异性单抗封闭喉癌细胞表面的α6、β4后可抑制肿瘤细胞的黏附与浸润。
Objective: To investigate the expression of integrin α6 and β4 in human laryngeal carcinoma cells Hep 2 and AMC HN 8 and their effects on the adhesion of tumor cells in vitro and their mechanisms. Methods: The expression of integrin α6 and β4 in Hep 2 and AMC HN 8 human laryngeal carcinoma cell lines was detected by flow cytometry. Adhesion inhibition test was performed on laryngeal carcinoma cells using the α6 and β4 specific monoclonal antibodies. Infiltration experiment. Results: The positive cells of integrin α6 and β4 were higher in Hep 2 and AMC HN 8 cell lines, respectively (45.73 ± 2.31)%, (25.76 ± 6.36) % And (45.5 ± 15.52)%, (84.5 ± 16.54)%, respectively. α6 specific monoclonal antibody had obvious adhesion inhibition on Hep 2 cell line (P0.05), β4 specific monoclonal antibody had no obvious adhesion inhibition (P0.05), anti-α6 plus anti-β4 adhesion (P <0.01). Anti-α6 or anti-β4 inhibited the adhesion of AMC HN 8 cells (all P <0.05), and the inhibition of anti-α6 and anti-β4 was more Obviously (P <0.01). Conclusion: Integrin α6 and β4 play an important role in the adhesion and invasion of laryngeal carcinoma cells. The use of α6, β4 specific monoclonal antibody blocking the surface of laryngeal carcinoma cells α6, β4 can inhibit tumor cell adhesion and infiltration.