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应用流式细胞术和免疫荧光染色技术，对４８例正常乳腺、乳腺囊性增生病和囊性增生癌变的石蜡包埋组织行ＤＮＡ倍体和癌基因ｃ－ｅｒｂＢ－２、抑癌基因Ｐ５３蛋白表达测定，结果表明，从正常乳腺组织到囊性增生癌变的发展过程中，ＤＩ值和ＳＰＦ值以及ＤＮＡ异倍体出现率呈明显增高趋势。癌变组织的。－ｅｒｂＢ－２和Ｐ５３表达显著增高，并与ＳＰＦ和ＤＮＡ异倍体率的增加正相关。提示乳腺囊性增生病的多多数（ＤＩ、ＳＰＦ、ｃ－ｅｒｂＢ－２、Ｐ５３）分析呈阳性，临床应按早期癌处理。
Flow cytometry and immunofluorescence staining techniques were used to analyze DNA ploidy, oncogene c-erbB-2 and tumor suppressor gene P53 protein in paraffin-embedded tissues of 48 normal breast, breast cystic hyperplasia and cystic hyperplasia. Expression assays showed that from the normal breast tissue to the development of cystic hyperplasia, the DI value and SPF value and the frequency of DNA aneuploidy showed a significant increase. Cancerous tissue. The expression of -erbB-2 and P53 was significantly increased and positively correlated with the increase of SPF and DNA heteroploidy. Prompt analysis of breast cystic hyperplasia majority (DI, SPF, c-erbB-2, P53) analysis was positive, clinical treatment should be based on early cancer.