论文部分内容阅读
硫撑双乙酸Thiodiglycollic acid(以下简称TdGA)是正常人体代谢产物,也是氯烯烃在机体内的主要最终产物之一。它亲水性强,从水溶液中萃取较困难,在担体上的吸附力亦很强。Muller曾用气相色谱氢焰检测器对其进行测试并用质谱仪分析鉴定之。从得到的尿样分析色谱图看(图1),低含量样品的特征峰较难辨认,氢焰的溶剂峰及尿中杂峰几乎将其淹没。为此,我们选用火焰光度检测器进行复杂生物试样分析,以建立预处理简便、选择性高、特征峰清晰和灵敏度能满足正常含量检测的方法。
Thiodiglycollic acid (hereinafter referred to as TdGA) is a normal human metabolite and is also one of the major end products of chlorinated olefins in the body. It is hydrophilic, more difficult to extract from aqueous solutions, and has a strong adsorption force on the support. Muller used a gas chromatography hydrogen flame detector to test it and analyze it by mass spectrometry. From the obtained urine sample chromatogram (Fig. 1), the characteristic peak of the low content sample is hard to recognize, and the peak of the hydrogen flame and the peak of the urine almost submerge it. To this end, we use flame photometric detector for complex biological sample analysis, in order to establish a simple pretreatment, high selectivity, clear peak characteristics and sensitivity to meet the normal content detection methods.