论文部分内容阅读
提出一种测定新药溴莫普林（ＢＤＰ）的微分吸附计时电位法。在Ｍｉｃｈａｅｌｉｓ（ＭＣＬ）缓冲液中（ｐＨ４．０）溴莫普林于－１．３３Ｖ左右产生一灵敏的微分吸附计时电位溶出峰．利用该峰测定ＢＤＰ的线性范围为２．０×１０－８～３．０×１０－７ｍｏｌ／Ｌ，吸附富集３ｍｉｎ检测下限为５．０×１０－９ｍｏｌ／Ｌ。探讨了影响灵敏度的主要因素，用循环伏安法，脉冲极谱等多种电化学方法研究了该体系的电化学行为和电极机理，认为所研究体系属于有吸附性的不可逆还原过程，电极反应的电子转移数为２，参与电极反应的质子数为２。用该方法测定了血清和尿样中ＢＤＰ的含量。
A new method for the determination of the new drug Buprelin (BDP) differential adsorption chronopotentiometry. Brimoprene in Michaelis (MCL) buffer (pH 4.0) produced a sensitive differential adsorption time-resolved peak at -1.33V. Using this peak, the linear range of BDP was 2.0 × 10-8 ~ 3.0 × 10-7mol / L, and the detection limit was 5.0 × 10-9mol / L for 3min. The main factors influencing the sensitivity were discussed. The electrochemical behavior and electrode mechanism of the system were studied by electrochemical methods such as cyclic voltammetry and pulsed polarography. It is concluded that the system under study belongs to an irreversible reduction process with adsorption. Electrode reaction Of the electron transfer number is 2, the number of protons involved in the electrode reaction is 2. The method was used to determine the content of BDP in serum and urine samples.