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目的研究人类端粒酶逆转录酶(hTERT)在嗜铬细胞瘤中的表达及其作为预测生物学行为标志物的价值。方法采用原位杂交方法测定hTERT mRNA在45例嗜铬细胞瘤和副神经节瘤和9例正常肾上腺髓质中的表达;免疫组织化学方法检测hTERT蛋白的表达水平。结果hTERT mRNA在5例恶性(5/7)和5例可疑恶性(5/7)肿瘤中表达,表达均高于良性肿瘤(3/31),差异有统计学意义(P<0.01);hTERT蛋白的表达在良性(3/31)和可疑恶性(6/7)以及恶性(5/7)差异有统计学意义(P<0.01);9例正常肾上腺髓质细胞中未发现hTERT mRNA和蛋白的表达;肿瘤组织hTERT蛋白和mRNA的表达存在正相关性(r=0.951,P<0.01);hTERT蛋白和mRNA的表达与临床因素,如性别、肿瘤大小和血压无关联。结论hTERT mRNA和蛋白检测对良、恶性嗜铬细胞瘤和副神经节瘤的鉴别诊断有重要临床意义。
Objective To study the expression of human telomerase reverse transcriptase (hTERT) in pheochromocytoma and its value as a predictor of biological behavior. Methods The expression of hTERT mRNA in 45 cases of pheochromocytoma and paraganglioma and 9 cases of normal adrenal medulla was determined by in situ hybridization. The expression of hTERT protein was detected by immunohistochemistry. Results The expression of hTERT mRNA in 5 cases of malignant (5/7) and 5 cases of suspected malignant (5/7) tumors was higher than that of benign tumor (3/31) (P <0.01). HTERT The expression of hTERT mRNA was significantly higher in benign (3/31), suspicious (6/7) and malignant (5/7) (P <0.01), but not in normal adrenal medulla (R = 0.951, P <0.01). The expression of hTERT protein and mRNA was not associated with clinical factors such as gender, tumor size and blood pressure. Conclusion The detection of hTERT mRNA and protein has important clinical significance in the differential diagnosis of benign and malignant pheochromocytoma and paraganglioma.