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观察EGF－PE40重组毒素对喉鳞癌细胞的毒性作用，为寻找新型的治疗头颈鳞癌的生物制剂奠定基础。应用MTT比色法测定EGF－PE40重组毒素的生物学活性。并在光镜下观察Hep－2细胞形态变化。确定MTT比色法中Hep－2细胞浓度为2×104／well。选择10％SDS－0．01mol／LHCI作为MTT结晶的溶解液，当EGF－PE40重组毒素为0．53ng／ml时，50％的Hep－2细胞死亡，在一定范围内，随重组毒素增加，细胞损伤加重。
To observe the toxic effect of EGF-PE40 recombinant toxin on laryngeal squamous cell carcinoma (LSCC) and lay the foundation for finding a new biological agent for the treatment of squamous cell carcinoma of the head and neck. The biological activity of EGF-PE40 recombinant toxin was determined by MTT assay. The morphological changes of Hep-2 cells were observed under light microscope. The concentration of Hep-2 cells in MTT assay was determined to be 2 × 10 4 / well. 50% of Hep-2 cells died when EGF-PE40 recombinant toxin was 0.53ng / ml when 10% SDS-0.01mol / LHCI was chosen as the solution of MTT crystal. Within a certain range, with the increase of recombinant toxin, Increased cell damage.