论文部分内容阅读
目的 观察幽门螺杆菌 (HP)阳性的胃溃疡病人在溃疡愈合前后胃上皮细胞凋亡及HP检测的情况。方法 采用检测DNA裂解的末端脱氧核苷酸转移酶 (TdT)介导的dUTP缺口末端标记法 (TUNEL) ,在原位标记凋亡的胃上皮细胞。结果 溃疡愈合后胃上皮细胞凋亡指数下降 ,与愈合前相比两者间有显著性差异 (P <0 0 5 )。溃疡愈合后HP全部阴转 ,HP的检出密度与胃上皮细胞凋亡指数之间呈正相关性 (r=0 .80 ,P <0 0 5 )。结论 HP在体内能诱导胃上皮细胞凋亡 ,导致溃疡的形成
Objective To observe the apoptosis of gastric epithelial cells before and after ulcer healing in patients with Helicobacter pylori (HP) -positive gastric ulcer and the detection of HP. Methods Apoptotic gastric epithelial cells were labeled in situ using terminal deoxynucleotidyl transferase (TdT) -mediated dUTP nick end labeling (TUNEL) assay. Results After gastric ulcer healed, the apoptosis index of gastric epithelial cells decreased, which was significantly different from that before healing (P <0.05). After healing of ulcer, all of HP was negative, and there was a positive correlation between HP detection density and gastric epithelial cell apoptosis index (r = 0.80, P <0.05). Conclusion HP can induce gastric epithelial cell apoptosis in vivo, resulting in the formation of ulcer