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以 3龄家蚕为供食对象 ,用洒过转苏云金杆菌 (Bacillusthuringiensis,Bt)基因水稻纯合品系KMD2生米粉或熟米粉的桑叶喂养 ,研究Bt抗虫水稻稻米对家蚕生长发育及中肠亚显微结构的影响 ,以明确生米粉与熟米粉之间的差异。结果发现 ,用前者喂养的家蚕体重、熟蚕数、结茧数、全茧量和茧层量均明显低于后者和对照 ,熟蚕整齐度明显迟于后者和对照 ;而后者与对照之间无明显差异。进一步的病理切片电镜分析表明 ,用前者喂养的家蚕 ,其中肠细胞亚显微结构发生了明显的变化 ,杯形细胞和圆筒形细胞的微绒毛明显变短、变粗 ,线粒体和粗面型内质网的数量显著下降 ;而后者与对照之间无显著差异。由此表明 ,经蒸煮后Bt水稻稻米中的杀虫蛋白发生变性 ,失去活性 ,进而丧失致毒能力。
The third instar silkworms were fed on mulberry leaves of mulberry leaves spiked with KMD2 or Bt rice, and the effects of Bt insect-resistant rice on the growth and development of silkworm (Bombyx mori) The effect of microstructure to clarify the difference between raw and cooked rice flour. The results showed that the body weight, number of mature silkworm, cocoon number, cocoon amount and cocoon layer of the silkworm fed with the former were significantly lower than those of the latter and the control, while that of the silkworm was significantly later than the control and the control No significant difference between. Further analysis of pathological sections showed that the sub-microstructure of intestinal cells changed obviously in the silkworms fed with the former, and the microvilli of goblet cells and cylindrical cells were obviously shorter and thicker. The mitochondria and rough surface type The number of endoplasmic reticulum decreased significantly, while there was no significant difference between the latter and the control. This shows that after cooking Bt rice in rice insecticidal protein denaturation, loss of activity, and thus loss of virulence.