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目的利用双通道放电等离子体反应装置,研究常压空气等离子体对液相体系中金黄色葡萄球菌的杀灭规律和机制。方法观察等离子体放电参数对金黄色葡萄球菌杀灭的剂量效应。从化学(羟基自由基)、物理(水体温度、紫外光)和菌体微观形态(透射电镜)等角度综合分析等离子体场中各活性物质的杀灭作用。结果常压空气等离子体液相杀菌效果与放电时间呈正相关关系。当电极距液面间距为1 mm,放电处理10 min时,杀灭率达99.91%。透射电镜技术观察金黄色葡萄球菌菌体形态和超微结构,发现杀菌后菌体变得相对模糊,出现明显变形、破裂甚至穿孔现象,细胞质流向周围介质。杀菌过程中羟基自由基起主要作用,而紫外光杀菌效应及热力杀菌效应仅起协同作用。结论该装置能实现对液相环境金黄色葡萄球菌杀灭,在等离子体场的强烈作用下细菌细胞发生破损是造成细菌死亡的主要原因。
OBJECTIVE To study the killing mechanism and mechanism of Staphylococcus aureus in liquid phase system by atmospheric air plasma using a dual-channel discharge plasma reactor. Methods The dose effects of plasma discharge parameters on the killing of Staphylococcus aureus were observed. The killing effect of each active substance in the plasma field was comprehensively analyzed from the aspects of chemical (hydroxyl free radical), physical (water temperature, ultraviolet light) and microscopic morphology of cell (transmission electron microscope). Results The atmospheric bactericidal effect of atmospheric air plasma showed a positive correlation with discharge time. When the electrode distance from the liquid surface is 1 mm, discharge treatment 10 min, the killing rate of 99.91%. Transmission electron microscopy was used to observe the morphology and ultrastructure of Staphylococcus aureus. It was found that the bacteria became obscure, with obvious deformation, rupture or perforation, and the cytoplasm flowed to the surrounding medium. Hydroxyl radicals play a major role in the sterilization process, while the bactericidal effect of ultraviolet light and the heat sterilization effect only play a synergistic effect. Conclusion The device can kill Staphylococcus aureus in liquid environment. Bacterial cell damage is the main cause of bacterial death under the strong action of plasma field.