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目的探讨慢病毒介导碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)基因修饰的骨髓基质细胞(bone marrow stromal cells,BMSCs)对脑梗死后血管新生的影响。方法利用慢病毒载体介导bFGF基因修饰BMSCs,获得稳定转染bFGF的BMSCs。脑梗死后1d立体定向移植至梗死灶周围。在MCAO术前及术后1、3、7、14 d进行神经功能评分。术后14 d股静脉注射异硫氰酸荧光素右旋糖酐(FITCdextran)标记微血管,结合共聚焦显微镜和3D Doctor 3.5版软件分析梗死灶周围区微血管的直径、面积及血管分支数目。结果 bFGF-BMSCs组术后3 d神经功能恢复明显优于对照组与BMSCs组(P<0.05),BMSCs组和bFGF-BMSCs组术后7 d及14 d mNSS评分显著低于对照组(P<0.05),而且bFGF-BMSCs组神经功能恢复好于BMSCs组(P<0.05)。bFGF-BMSCs组微血管直径、分支数目及面积显著性高于对照组(P<0.01)和BMSCs组(P<0.05)。结论 bFGF修饰的BMSCs能更好地促进脑梗死后神经功能恢复及血管新生。
Objective To investigate the effect of leukemia-induced bone marrow stromal cells (BMSCs) modified by basic fibroblast growth factor (bFGF) on angiogenesis after cerebral infarction. Methods BMSCs were modified by lentiviral vector mediated bFGF gene to obtain BMSCs stably transfected with bFGF. Stent stereotactic transplantation 1d after infarction around cerebral infarction. Neurological scores were assessed before and at 1, 3, 7 and 14 days after MCAO. Fourteen days after operation, FITC dextran sulfate (FITCdextran) was injected into the femoral vein. The diameter, area and number of branches of blood vessels around the infarct area were analyzed by confocal microscopy and 3D Doctor 3.5 software. Results The neural function recovery of bFGF-BMSCs group was significantly better than that of BMSCs group and BMSCs group (P <0.05). The mNSS scores of BMSCs group and bFGF-BMSCs group at 7 and 14 d after operation were significantly lower than those of control group (P < 0.05). Moreover, the neurological function recovery of bFGF-BMSCs group was better than that of BMSCs group (P <0.05). The microvessel diameter, branch number and area in bFGF-BMSCs group were significantly higher than that in control group (P <0.01) and BMSCs group (P <0.05). Conclusion bFGF-modified BMSCs can better promote neurological function and angiogenesis after cerebral infarction.