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在酸性条件下,Ce(Ⅳ)-罗丹明6G发光体系有较强的化学发光,盐酸多西环素及土霉素的存在能抑制其发光。且化学发光信号的减小值(ΔI)与盐酸多西环素及土霉素的浓度在一定的范围内有较好的线性关系。据此结合流动注射技术,建立了快速测定盐酸多西环素及土霉素的流动注射化学发光分析新方法。优化了影响化学发光的各种因素。结果表明在优化的条件下,盐酸多西环素及土霉素的线性范围分别是1.0×10-7~1.0×10-4g/mL,1.0×10-6~1.0×10-4g/mL,测定的检出限为5.0×10-8g/mL及2.7×10-7g/mL,对1.0×10-6g/mL的盐酸多西环素及1.0×10-5g/mL的盐酸土霉素分别进行了9次平行测定,其相对标准偏差分别为1.4%、1.6%。方法已用于盐酸多西环素及土霉素片剂的测定。
Under acidic conditions, Ce (Ⅳ) -rhodamine 6G luminescent system has strong chemiluminescence, and the presence of doxycycline hyclate and oxytetracycline can inhibit its luminescence. The decrease of chemiluminescence signal (ΔI) and the concentration of doxycycline hydrochloride and oxytetracycline in a certain range have a good linear relationship. Based on this, a new flow-injection chemiluminescence method for the determination of doxycycline hyclate and oxytetracycline hydrochloride was established. Various factors that affect chemiluminescence are optimized. The results showed that under the optimized conditions, the linear range of doxycycline hydrochloride and oxytetracycline was 1.0 × 10-7 ~ 1.0 × 10-4g / mL, 1.0 × 10-6 ~ 1.0 × 10-4g / mL, The limits of detection were 5.0 × 10 -8 g / mL and 2.7 × 10 -7 g / mL, respectively, for doxorubicin hydrochloride 1.0 × 10 -6 g / mL and oxytetracycline hydrochloride 1.0 × 10 -5 g / mL Nine parallel assays were performed with relative standard deviations of 1.4% and 1.6%, respectively. The method has been used for the determination of doxycycline hyclate and oxytetracycline tablets.