目的 :探讨巢蛋白 (nestin)在胚胎后期、新生期、成年三阶段的核酸、蛋白水平表达的变化 .方法 :取三个发育时期的胰腺 ,应用RT PCR、免疫组化的方法进行半定量、定位分析 .结果 :在胚胎后期、新生期、成年三阶段nestin在核酸、蛋白水平均有表达 ,阳性细胞主要分布在胰岛 ,在腺泡周围的外分泌中有少量分布 .结论 :随大鼠胰腺的生长发育 ,核酸、蛋白表达下调 .胰岛内nestin阳性细胞数也呈下降趋势 Objective: To investigate the changes of nestin expression in the three stages of embryonic embryo, neonatal and adult.Methods: The pancreas of three developmental stages were collected and subjected to semi-quantitative RT-PCR and immunohistochemistry Location analysis showed that nestin was expressed in the three stages of embryo, newborn and adult, and the positive cells were mainly distributed in the islets, with a small amount of exocrine secretion around the acinar.Conclusion: Growth and development, the expression of nucleic acid and protein were down-regulated.The number of nestin positive cells in islets also showed a downward trend