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以新疆塔里木盆地的光果甘草(Glycyrrhiza glabra)和胀果甘草(G.inflate)为研究对象,采用变性梯度凝胶电泳(PCR-DGGE)技术研究甘草内生真菌的多样性及群落结构。结果显示,光果甘草和胀果甘草间及同一种类不同组织间的内生真菌多样性和群落结构均存在明显差异。其中,胀果甘草根(B1)的内生真菌多样性最丰富,光果甘草果(L4)的内生真菌多样性最差。对DGGE条带进行回收测序,共获得25条序列,归于枝顶孢属(Acremonium)、绿僵菌属(Metarhizium)、绿僵虫草属(Metacordyceps)、镰刀菌属(Fusarium)、链格孢属(Alternaria)、枝孢属(Cladosporium)、锤舌菌属(Leotiomycetes)、链格孢属(Alternaria)、帚枝霉属(Sarocladium)、假裸囊菌属(Pseudogymnoascus)、曲霉属(Aspergillus)11个真菌属,其中链格孢属为优势菌属,占总数的32%。甘草根、茎、叶、果、皮组织的内生真菌存在丰富的多样性,其中根与茎组织多样性最丰富。研究结果表明,新疆塔里木盆地药用植物甘草蕴藏着丰富的内生真菌资源,可作为一种较理想的分离内生真菌的植物来源。
Glycyrrhiza glabra and G.inflate in the Tarim Basin of Xinjiang were used to study the diversity and community structure of licorice endophytic fungi by denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that there were significant differences in the diversity and community structure of endophytic fungi between Glycyrrhiza glabra and Glycyrrhiza inflata and the same species. Among them, the endophytic fungi diversity of Glycyrrhiza inflata (B1) was the most abundant, and the endophytic fungi diversity of Glycyrrhiza glabra (L4) was the worst. The sequences of DGGE bands were recovered and sequenced, and 25 sequences were obtained, belonging to Acremonium, Metarhizium, Metacordyceps, Fusarium, Alternaria, Cladosporium, Leotiomycetes, Alternaria, Sarocladium, Pseudogymnoascus, Aspergillus 11 A fungi genus, which Alternaria is dominant genus, accounting for 32% of the total. Endophytic fungi in roots, stems, leaves, fruits and barks of licorice were rich in diversity, of which the root and stem tissues were the most abundant. The results show that medicinal plant licorice in the Tarim Basin in Xinjiang is rich in endophytic fungi and can be used as an ideal plant source for isolation of endophytic fungi.