LTR位于HIV前病毒同DNA基因组的两末端,Gag基因位于5′端LTR的下游。LTR对转录的调控作用主要是由5′端LTR进行的。LTR具有启动子的作用,在结构上具有真核启动子的典型特征。另外,HIV-1 Gag蛋白还有能够自我装配成病毒样粒子(virus like particle,VLP)并从细胞中释放出来的重要特性。 我们利用痘苗病毒表达系统,探索了在细胞质环境中LTR中的多种功能结构对Gag蛋 The LTR is located at both ends of the HIV provirus and DNA genomes and the Gag gene is located downstream of the 5 ’LTR. The regulatory effect of LTR on transcription is mainly by the 5 ’LTR. LTR has the function of a promoter, which has the structural characteristics of a typical eukaryotic promoter. In addition, HIV-1 Gag proteins have important properties that self-assemble into virus-like particles (VLPs) and are released from the cells. Using vaccinia virus expression systems, we explored the multiple functional structures of LTRs in the cytoplasmic environment of Gag eggs