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目的探讨中国新疆地区维吾尔族慢性阻塞性肺疾病(简称慢阻肺)与解整连蛋白和金属蛋白33(ADAM33)基因V4及F+1位点单核苷酸多态性的相关性。方法采用限制性片段长度多态性方法分析初次确诊的维吾尔族慢阻肺患者100例(慢阻肺组)与同期健康体检者140例(对照组)ADAM33 V4和F+1位点基因型,对所有研究对象进行肺功能检查和慢阻肺流行病学调查,对慢阻肺组患者病情进行慢阻肺综合评估。结果初次就诊的维吾尔族慢阻肺患者经慢阻肺综合评估,A组、B组和C组患者分别占22%、35%和30%。慢阻肺组和对照组间ADAM33基因V4和F+1位点基因型构成比差异均无统计学意义(P>0.05),两组ADAM33基因V4和F+1位点的3种基因型患者FEV1%pred和FEV1/FVC均值比较差异无统计学意义(P>0.05)。慢阻肺组ADAM33基因V4、F+1位点不同基因型与慢阻肺综合评估之间无显著相关性(P>0.05)。SHEsis在线软件对V4、F+1位点进行单体型分析结果显示4种单体型在慢阻肺组和对照组比较差异无统计学意义(P>0.05)。结论初步就诊的维吾尔族慢阻肺患者中,慢阻肺综合评估B组及C组比例较大。ADAM33基因V4、F+1位点多态性与新疆维吾尔族慢阻肺不相关,与肺功能及病情程度不相关,不增加维吾尔族患者慢阻肺易感性。
Objective To investigate the association of Uygur chronic obstructive pulmonary disease (COPD) with single nucleotide polymorphisms at V4 and F + 1 loci of ADAM33 gene in Xinjiang region of China. Methods A total of 100 ADHD patients with chronic obstructive pulmonary disease (COPD) and 140 healthy subjects (control group) with ADAM33 V4 and F + 1 loci were analyzed by restriction fragment length polymorphism (PCR-RFLP) Lung function tests and COPD epidemiological survey of all the subjects were conducted, and COPD patients were evaluated for COPD. Results The first visit of Uygur patients with chronic obstructive pulmonary disease by comprehensive assessment of COPD, A, B and C patients accounted for 22%, 35% and 30%. There were no significant differences in the genotypes of ADAM33 gene V4 and F + 1 between COPD group and control group (P> 0.05). There were no significant differences in the genotypes of ADAM33 gene among the three groups There was no significant difference in mean FEV1% pred and FEV1 / FVC (P> 0.05). There was no significant correlation between the different genotypes of ADAM33 gene V4, F + 1 locus and the comprehensive assessment of COPD in COPD group (P> 0.05). The haplotype analysis of V4 and F + 1 sites by SHEsis online software showed that there was no significant difference among the four haplotypes in COPD group and control group (P> 0.05). Conclusion Among the Uygur COPD patients who initially treated, COPD patients in group B and group C had a larger proportion. Polymorphism of V4 and F + 1 loci in ADAM33 gene was not related to COPD in Xinjiang Uygur, but not to pulmonary function and severity, and did not increase COPD susceptibility in Uygur patients.