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目的探讨DNA损伤修复基因XRCC1 Arg194Trp位点多态性与结直肠癌易感性的关系。方法选取120例结直肠癌患者与120例正常对照者进行对比研究。取外周血提取DNA,采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术对XRCC1 Arg194Trp基因多态性进行检测分析,比较不同基因型与结直肠癌易感性的关系。结果 2组观察对象在年龄、性别、吸烟、饮酒、饮食特点等常见暴露因素方面的差异均无统计学意义(P>0.05),变异基因型Arg/Trp+Trp/Trp出现频率在2组观察对象中分别为30.00%和24.17%,差异无统计学意义(P>0.05)。结论 XRCC1 Arg194Trp位点多态性与结直肠癌的易感性并无显著相关性。
Objective To investigate the relationship between DNA damage repair gene XRCC1 Arg194Trp polymorphism and the susceptibility to colorectal cancer. Methods 120 patients with colorectal cancer and 120 normal controls were compared. DNA was extracted from peripheral blood and the polymorphism of XRCC1 Arg194Trp was detected by PCR-RFLP. The relationship between different genotypes and susceptibility to colorectal cancer was analyzed. Results There was no significant difference in the common exposure factors such as age, gender, smoking, drinking, diet characteristics between the two groups (P> 0.05). The frequency of Arg / Trp + Trp / Trp in the two groups was observed in two groups The subjects were 30.00% and 24.17%, respectively, with no significant difference (P> 0.05). Conclusion There is no significant correlation between the polymorphism of XRCC1 Arg194Trp locus and the susceptibility to colorectal cancer.