论文部分内容阅读
目的 :观察肝缺血再灌注损伤时脂质过氧化的动态变化和川芎嗪的干预作用 ,并探讨其机制。方法 :复制健康家兔肝缺血再灌注损伤模型 ,随机分为对照组 (n =10 )和川芎嗪组 (n =10 )。连续观察缺血前、缺血 2 5min、再灌注 2 5min、6 0min及 12 0min时血浆中丙二醛 (MDA)含量、超氧化物歧化酶 (SOD)、黄嘌呤氧化酶 (XO)及谷丙转氨酶 (ALT)活性的动态变化 ,并观察川芎嗪对不同时限上述指标的影响。结果 :随缺血再灌注时间的延长 ,对照组的MDA、XO和ALT呈阶梯式的明显升高 ,SOD呈逐渐显著降低的变化 ;川芎嗪组的上述指标在再灌注的各时限与对照组比较差异均有显著或非常显著性意义 (P <0 .0 5或P <0 .0 1)。结论 :肝缺血再灌注损伤时脂质过氧化反应呈进行加重的变化 ;川芎嗪能抑制氧自由基的生成 ,增强对氧自由基的清除 ,从而起着良好的抗脂质过氧化作用
OBJECTIVE: To observe the dynamic changes of lipid peroxidation and the effects of ligustrazine on hepatic ischemia-reperfusion injury and to explore its mechanism. Methods: A model of hepatic ischemia-reperfusion injury in healthy rabbits was duplicated and randomly divided into control group (n = 10) and ligustrazine group (n = 10). The contents of malondialdehyde (MDA), superoxide dismutase (SOD), xanthine oxidase (XO) and glutathione peroxidase in plasma were measured before ischemia, 25 min ischemia, 25 min reperfusion, 60 min and 120 min (ALT) activity changes, and observe the impact of ligustrazine on the above indicators of different time. Results: The MDA, XO and ALT in the control group increased significantly with the increase of ischemia-reperfusion time and the SOD decreased gradually. The above indexes in the ligustrazine group were significantly higher than those in the control group The difference was significant or very significant (P <0.05 or P <0.01). CONCLUSION: The changes of lipid peroxidation in liver ischemia-reperfusion injury are aggravating. Ligustrazine can inhibit the formation of oxygen free radicals, enhance the scavenging of oxygen free radicals, and thus play a good anti-lipid peroxidation