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研究了水相中阳离子交换树脂SAC001×7对阿卡波糖的吸附平衡、吸附热力学及吸附动力学,探讨了运用SAC001×7从发酵液中分离阿卡波糖的可行性。吸附平衡实验表明,SAC001×7吸附阿卡波糖的最大平衡吸附容量在研究范围内随温度升高而增大,在313 K时达到951.5 mg g 1,且吸附等温线符合Langmuir模型。计算了阿卡波糖吸附热力学函数,ΔG<0,ΔS>0,ΔH>0,表明SAC001×7吸附阿卡波糖是一个吸热过程,升高温度促进吸附。吸附动力学实验表明,90%以上的阿卡波糖吸附发生在前40 min以内,120 min达到吸附平衡,阿卡波糖交换速率受颗粒内扩散限制。在考察浓度范围内,内扩散速率常数随阿卡波糖初始浓度增大而增大,受温度的影响较小。
The adsorption equilibrium, adsorption thermodynamics and adsorption kinetics of cation exchange resin SAC001 × 7 on acarbose in aqueous phase were studied. The feasibility of separating acarbose from fermentation broth by using SAC001 × 7 was discussed. Adsorption equilibrium experiments showed that the maximum equilibrium adsorption capacity of acacarbose adsorbed by SAC001 × 7 increased with the increase of temperature in the range of the study, reached 951.5 mg g 1 at 313 K, and the adsorption isotherm fitted the Langmuir model. The thermodynamic functions of acarbose adsorption were calculated, ΔG <0, ΔS> 0, ΔH> 0, indicating that adsorption of acarbose by SAC001 × 7 is an endothermic process and the temperature is increased to promote the adsorption. Adsorption kinetics experiments showed that more than 90% of acarbose adsorption occurred within the first 40 min and reached adsorption equilibrium at 120 min, and the acarbose exchange rate was limited by intraparticle diffusion. Within the investigated concentration range, the internal diffusion rate constant increases with the initial concentration of acarbose and is less affected by the temperature.