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目的探究丙型肝炎患者血清中自身抗体的检测与临床意义,为临床提供参考经验。方法选取怀化市第一人民医院2012年3月～2013年3月所收治的72例丙型肝炎患者作为研究对象,感染患者血清采用荧光定量聚合酶链反应检测HCV-RNA,用间接免疫荧光法检测抗核抗体(ANA),用ELSIA法检测抗-HCv抗体,用免疫印迹法检测抗线粒体AMA-M 2抗体,抗Sp 100抗体、抗肝肾微粒体-1(LKM-1)抗体、抗肝特异性胞质1型(LC-1)抗体、抗Ro-52抗体等。结果 72例患者中,自身抗体阳性22例,总阳性率为30.56%。其中25例HCV-RNA(+)患者中,自身免疫抗体检出率为44.0%(11/25);47例HCV-RNA(-)患者中,自身免疫抗体检出率为21.3%(10/47),两组患者自身抗体检查率差异具有统计学意义(P<0.05)。结论 HCV感染可诱发机体自身免疫反应,产生多种自身抗体,其中HCV-RNA(+)患者效果更优。
Objective To investigate the detection and clinical significance of serum autoantibodies in patients with hepatitis C and provide reference for clinical practice. Methods Seventy-two patients with hepatitis C who were treated in First People’s Hospital of Huaihua City from March 2012 to March 2013 were selected as experimental subjects. HCV-RNA was detected by fluorescence quantitative polymerase chain reaction in sera of infected patients, and indirect immunofluorescence assay Anti-nuclear antibody (ANA) was detected by ELISA. Anti-HCV antibody was detected by ELSIA. Anti-mitochondrial AMA-M2 antibody, anti-Sp100 antibody and anti-LKM-1 antibody were detected by Western blot Liver-specific cytoplasmic type 1 (LC-1) antibody, anti-Ro-52 antibody and the like. Results Of 72 patients, autoantibodies were positive in 22 cases, the total positive rate was 30.56%. Among the 25 patients with HCV-RNA (+), the autoimmune antibody was detected in 44.0% (11/25) of the 25 HCV-RNA (+) patients, and 21.3% 47). The difference of autoantibodies between the two groups was statistically significant (P <0.05). Conclusion HCV infection can induce autoimmune reactions in the body and produce a variety of autoantibodies, of which HCV-RNA (+) patients are more effective.