标本溶血对ELISA检测乙肝表面抗原的影响

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目的 分析溶血标本采用ELISA法检测HBsAg时对结果的影响.方法 选取HbsAg阳性和HbsAg阴性标本各50例,采用冰冻法使这100例标本溶血.溶血标本采用酶联免疫吸附试验(ELISA)检测HBsAg,酶标仪判读结果.结果 HbsAg阴性溶血标本检测HbsAg的假阳性率为30%,而HbsAg阳性溶血标本检测HbsAg的假阴性率为8%.结论 溶血标本采用ELISA法检测HbsAg时容易产生假阴性或假阳性.
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