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利用分离、纯化的玉米矮花叶病毒及其结构蛋白质制剂,获得了特效免疫血清,采用含十二烷基硫酸钠(SDS)的琼脂双向免疫扩散法,以及病毒与细菌凝集反应(ABB)试验和酶联免疫吸附(ELISA)测定法进行了病毒免疫诊断。结果表明,ELISA法和法的高度灵敏性保证了对玉米该病害进行免疫诊断的可靠性。玉米矮花叶病毒引起的病害主要是降低玉米、高粱和苏丹草的籽粒及绿色体产量。在自然条件下,人们通常认为多年野生禾本科植物约翰逊草(S.halepense)是玉米矮花
Using the isolated and purified maize dwarf mosaic virus and its structural protein preparations, the specific immune serum was obtained. The agar two-way immunodiffusion method with sodium dodecyl sulfate (SDS) and the virus and bacterial agglutination reaction (ABB) test And enzyme-linked immunosorbent assay (ELISA) virus immunoassay. The results show that the high sensitivity of the ELISA method and the method ensures the reliability of immunodiagnosis of the disease in maize. The diseases caused by maize dwarf mosaic virus are mainly to reduce the grain and green body yield of corn, sorghum and sudangrass. Under natural conditions, it is generally accepted that S.halepense, a wild graminaceous plant,