目的:探讨非小细胞肺癌患者癌组织中长链非编码RNA LINC01614表达及临床意义。方法:选取2016年1月至2017年12月在鞍钢集团总医院行手术切除的75例原发性NSCLC患者癌组织为研究对象，并选取相对应癌旁组织(距肿瘤边缘>5 cm)作为对照，回顾性分析NSCLC相关lncRNA的表达，用实时荧光RT-PCR法检测组织中LINC01614水平，分析LINC01614的表达与NSCLC患者临床病理特征的相关性，绘制受试者工作特征曲线确定LINC01614诊断NSCLC的效能参数；采用Kaplan-Meier曲线进行生存分析。结果:对16个样本进行lncRNA微阵列分析(癌组织和癌旁组织各8个)，NSCLC患者中发现3 261个lncRNA表达水平显著不同，其中1 205个表达上调，2 056个表达下调。同时，LINC01614为最紊乱的lncRNA，在癌组织中表达上调，是癌旁组织的22.92倍。TNM分期越高、组织分化程度越低、发生淋巴结转移患者，LINC01614相对表达量越高。曲线下面积为0.788(95%n CI：0.710~0.821)，其诊断敏感度为58.62%，特异度为87.24%；以LINC01614相对表达量26.14作为截断值，将NSCLC患者分为高表达组(26例)和低表达组(49例)，高表达组和低表达组的总生存期分别为22.2个月(95%n CI：20.1~34.6)和38.5个月(95%n CI：25.3~45.5)，高表达组的生存时间明显低于低表达组(n Z＝10.248，n P<0.05)。多元COX比例风险逐步回归分析显示LINC01614高表达为非小细胞肺癌患者预后的独立危险因素(n HR＝2.25，95%n CI：1.30~3.90)。n 结论:LINC01614在NSCLC组织中高表达，并与NSCLC的恶性程度有关，可能对NSCLC的预后判断具有一定的指导意义。“,”Objective:To investigate the expression and clinical significance of long non coding RNA LINC01614 in non-small cell lung cancer (NSCLC).Methods:From January 2016 to December 2017, 75 patients with primary NSCLC who underwent surgical resection in our hospital were selected as the cases, and the corresponding adjacent tissues (more than 5 from the tumor edge) were selected as control.The correlation between LINC01614 expression and clinicopathological characteristics of NSCLC patients was analyzed.ROC curve was drawn to determine the efficacy parameters of LINC01614 in the diagnosis of NSCLC.Kaplan Meier curve was used to analyze the survival analysis.Results:The expression levels of 3 261 lncRNAs were significantly different in NSCLC patients, including 1 205 up-regulated and 2 056 down-regulated.At the same time, LINC01614 was the most disordered lncRNA, and its expression was up-regulated in cancer tissues, 22.92 times higher than that in paracancerous tissues.The higher the TNM stage, the lower the degree of tissue differentiation and the occurrence of lymph node metastasis, the higher the relative expression of LINC01614.The area under the ROC curve was 0.788 (95% n CI: 0.710-0.821), with a diagnostic sensitivity of 58.62% and a specificity of 87.24%.With the relative expression of LINC01614 of 26.14 as the intercept, the overall survival of NSCLC patients in the high expression group (26 cases) and the low expression group (49 cases) was 22.2 months (95% n CI: 20.1-34.6) and 38.5 months (95% n CI: 25.3-45.5), respectively.The survival time of the high expression group was significantly lower than that of the low expression group (n Z=10.248, n P<0.05). Multivariate COX proportional hazards stepwise regression analysis showed that LINC01614 overexpression was an independent prognostic factor for NSCLC patients (n HR=2.25, 95%n CI: 1.30-3.90).n Conclusions:LINC01614 is highly expressed in NSCLC tissues, and is related to the malignant degree of NSCLC, which may have a certain guiding significance for the prognosis of NSCLC.