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目的探讨人工重组Clara细胞蛋白16(rhCC16)对放射性肺炎及肺纤维化的防护机制。方法将30只SD大鼠随机分成3组,对照组(n=10)、照射组(n=10)和rhCC16+照射组(n=10)。照射组和rhCC16+照射组均予单次全胸15 Gy剂量进行照射。3组均取SD大鼠肺组织进行HE、Masson染色;免疫组化、Western Blot及Real Time PCR检测白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、转化生长因子β(TGF-β)、Bax以及Bcl-2的表达;ELASA检测血清中IL-6、TNF-α和TGF-β水平;Western Blot、Real Time PCR以及MTT验证rhCC16对大鼠肺泡上皮细胞的保护作用。结果与对照组比较,照射组肺组织呈现明显的炎性细胞渗出,纤维病变明显,肺泡结构破坏,细胞凋亡增多,IL-6、TNF-α以及TGF-β表达水平增高;rhCC16干预处理后病变现象明显减轻,IL-6、TNF-α以及TGF-β的表达明显降低。结论 rhCC16可以抑制辐射诱导的炎症反应、细胞凋亡以及纤维病变,对放射性肺炎及肺纤维化具有防护作用。
Objective To investigate the protective mechanism of artificial recombinant Clara cell protein 16 (rhCC16) on radiation pneumonitis and pulmonary fibrosis. Methods Thirty SD rats were randomly divided into three groups: control group (n = 10), irradiation group (n = 10) and rhCC16 + irradiation group (n = 10). Irradiation group and rhCC16 + irradiation group were given a single full-thoracic radiation dose of 15 Gy. The lungs of SD rats were stained with HE and Masson in 3 groups, and the expressions of IL-6, TNF-α, The levels of IL-6, TNF-α and TGF-β in serum were detected by ELASA. The expressions of TGF-β, Bax and Bcl-2 were detected by Western Blot, Real Time PCR and MTT. Protective effects. Results Compared with the control group, the lung tissue of the irradiation group showed obvious inflammatory cell exudation, obvious fibrosis, destruction of alveolar structure, increased apoptosis and increased expression of IL-6, TNF-α and TGF-β; After the lesion was significantly reduced, IL-6, TNF-α and TGF-β expression was significantly reduced. Conclusion rhCC16 can inhibit radiation-induced inflammation, apoptosis and fibrosis, and has protective effects on radiation pneumonitis and pulmonary fibrosis.