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研究了超高效液相色谱-质谱检测器(UPLC-QDa)对γ-氨基丁酸的分析检测,建立了UPLC测定血液中γ-氨基丁酸的含量的分析方法。采用乙腈萃取血液样品,经BEH C18色谱柱(2.1×50 mm,1.7μm)分离,以0.1%甲酸水溶液(含0.5%乙酸胺):乙腈(95:5,V/V)为流动相,流速0.2 m L/min,质谱检测器(QDa)检测,正离子SIR模式,m/z=104进行定量,进样量1μL。对照品在0.5~10μg/m L浓度范围内与峰面积线性关系良好。3个添加试验的平均回收率分别为78.9%,87.6%,79.5%,RSD分别为1.2%,1.1%,2.1%。
The analysis of γ-aminobutyric acid by UPLC-QDa was studied, and the method of UPLC for the determination of γ-aminobutyric acid in blood was established. Blood samples were extracted with acetonitrile and separated on a BEH C18 column (2.1 × 50 mm, 1.7 μm). The mobile phase consisted of 0.1% formic acid in water (acetonitrile: 95: 5, V / V) 0.2 m L / min, mass spectrometry detector (QDa) detection, positive ion SIR mode, m / z = 104 for quantification, injection volume 1 μL. The reference substance has a good linear relationship with the peak area in the concentration range of 0.5 ~ 10μg / mL. The average recoveries of the three addition trials were 78.9%, 87.6% and 79.5%, respectively, with RSDs of 1.2%, 1.1% and 2.1%, respectively.