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应用PCR技术及Southernblot分子杂交检测87例胃癌患者胄液脱落癌细胞c－erbB－2扩增。结果显示：87例中有11例（12．64％）胃液c－erbB－2扩增阳性，其中进展期胄癌、乳头状腺癌及分化良好型胃癌阳性率分别显著高于早期胃癌、其它组织类型胃癌及分化不良型胃癌（P＜0．05与P＜0．01）；伴肝转移胃癌阳性率显著高于不伴肝转移者（P＜0．05）；伴淋巴结转移胃癌阳性率明显高于不伴淋巴结转移者（但P＞0．05）。该方法似可作为术前或胃镇检查前胃癌估计预后的一项指标。
PCR amplification and Southern blot hybridization were used to detect c-erbB-2 expansion in sputum cancer cells from 87 patients with gastric cancer. The results showed that there were 11 cases (12.64%) of gastric fluid c-erbB-2 amplification positive in 87 cases, among which the positive rate of advanced stage carcinoma of the palate, papillary adenocarcinoma and differentiated type of gastric carcinoma were significantly higher than those of early stage gastric carcinoma and others, respectively. Tissue type and poorly differentiated gastric cancer (P<0.05 and P<0.01); Positive rate of gastric cancer with liver metastasis was significantly higher than that without liver metastasis (P<0.05); Positive rate of gastric cancer with lymph node metastasis Significantly higher than those without lymph node metastasis (but P>0.05). This method may be used as an indicator of preoperative or gastric preoperative gastric cancer to estimate the prognosis.