论文部分内容阅读
目的：观察正常豚鼠耳蜗螺旋神经节钙调素的分布，因为作为真核细胞中最重要的Ｃａ２＋受体钙调素（ＣａＭ）在听觉径路上的分布和功能尚未见报道．方法：用健康白色豚鼠分成实验组和对照组，耳蜗脱钙后行石蜡包埋切片，采用免疫组化ＡＢＣ法定位螺旋神经节中的ＣａＭ免疫反应（ＣａＭ－ＩＲ）．结果：在耳蜗顶至底回螺旋神经节细胞中见ＣａＭ－ＩＲ分布但形态不一，或淡染或深染，或胞浆内或核及胞浆内均有．结论：说明了耳蜗螺旋神经节细胞内含ＣａＭ，并且细胞之间的功能状态可能不同，其ＣａＭ可能参与听觉信号的传导以及中枢对耳蜗的调抑功能，这种“上传下达”是通过ＣａＭ参与神经递质的合成与释放、轴浆运输完成的．
OBJECTIVE: To observe the distribution of calmodulin in the cochlear spiral ganglion of normal guinea pigs, as the distribution and function of the most important Ca2 + receptor calmodulin (CaM) in eukaryotic cells on the auditory pathways have not been reported yet. Methods: Healthy white guinea pigs were divided into experimental group and control group. The cochlea were decalcified and paraffin-embedded sections were obtained. The CaM-IR in the spiral ganglion was localized by immunohistochemical ABC method. Results: CaM-IR distribution was observed in the spiral ganglion cells from the top of the cochlea to the bottom of the spiral ganglion. However, the morphological changes of CaM-IR were not observed, either in light or dark staining, or in cytoplasm or nucleus and cytoplasm. CONCLUSIONS: It shows that CaM exists in the cochlear spiral ganglion cells and the functional status may be different among the cells. CaM may be involved in the conduction of auditory signals and the central inhibitory function of the cochlea. This “uploading” is mediated by CaM Synthesis and release of neurotransmitters, axoplasm transport completed.