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本研究采用15只成熟母羊,在繁殖季节,用含40mg醋酸氟孕酮的阴道海绵体同时引起动情期.12天以后除去海绵体,肌肉注射100IU PMSG,24小时后(即动情第一日)将动物随机分成三组:A组(5只羊)作对照.B组(5只羊)从动情期第七至二十三天皮下注射消炎痛100mg,一日三次.C组(5只羊)从动情期第七至十四天接受同样剂量的消炎痛治疗,作为消炎痛对子宫内膜PG浓度效应的对照.消炎痛停止后,立即将B、C组动物处死,除去产道.从黄体同侧子宫角至肉阜内膜组织(400mg)分成两标本分别称重,将其一用干冰冻后放塑料瓶中储于-20℃直至PG分析.对B组
In this study, 15 mature ewes were used to induce estrus in vaginal sponges containing 40 mg of fluprogesterone acetate during the breeding season. After 12 days, sponges were removed and 100 IU of PMSG was injected intramuscularly. After 24 hours Animals were randomly divided into three groups: Group A (5 sheep) as control, Group B (5 sheep) were given subcutaneous injections of indomethacin 100 mg three times a day from the seventh to the 23rd day of estrus Sheep) received the same dose of indomethacin treatment from the seventh to fourteenth days of the estrus as a control of the effect of indomethacin on endometrial PG concentration.After indomethacin ceased, the animals in groups B and C were immediately sacrificed to remove the birth canal The corpus luteum in the same side of the corpus luteum to the meat of the endometrial tissue (400mg) were divided into two specimens were weighed, the first with a dry ice placed in plastic bottles stored at -20 ℃ until PG analysis of the B group