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目的 :研究乙型肝炎病毒感染患者TTV重叠感染情况及初步探讨TTV的致病性。方法 :在ORF1区设计特异性引物建立巢式多聚酶链式反应 (PCR) ,检测乙型肝炎病毒感染患者血清中TTVDNA ,对PCR产物进行分子克隆和测序。结果 :献血员、慢性乙型肝炎病毒携带者、慢性乙型肝炎和慢性重型乙型肝炎患者TTVDNA阳性率分别为 10 %、11%、2 7%、4 8%。其中前两者与后两者比较均有显著差异 ,慢性乙型肝炎和慢性重型乙型肝炎患者相比也有显著差异 (P <0 .0 5 ) ,乙型肝炎病毒感染者中其HBV感染复制指标的阳性率在TTVDNA阳性与TTVDNA阴性组之间无明显差异 (P >0 .0 5 )。慢性乙型肝炎和慢性重型乙型肝炎患者的ALT和TBIL在TTVDNA阳性病人组与TTVDNA阴性病人组之间比较有显著性差异 (P <0 .0 5 ) ,TTVDNA阳性病人组明显高于TTVDNA阴性病人组。结论 :献血员中存在TTV感染 ,乙型肝炎病毒感染患者重叠感染TTV较常见。慢性乙型肝炎和慢性重型乙型肝炎患者TTVDNA阳性率明显高于献血员和慢性乙型肝炎病毒携带者的阳性率 ,提示TTV的重叠感染可能是HBV感染病情加重因素之一 ,TTV重叠感染对HBV的复制可能不存在相互作用。
Objective: To study the prevalence of overlapping TTV infection in patients with hepatitis B virus infection and to explore the pathogenicity of TTV. Methods: Nested polymerase chain reaction (PCR) was designed in ORF1 to detect TTVDNA in serum of patients with hepatitis B virus infection. The PCR products were cloned and sequenced. Results: The positive rates of TTVDNA in blood donors, carriers of chronic hepatitis B, chronic hepatitis B and chronic severe hepatitis B were 10%, 11%, 27% and 48% respectively. Among them, there was a significant difference between the former two and the latter two, there was also a significant difference between chronic hepatitis B and chronic severe hepatitis B patients (P <0.05), HBV infection in patients with hepatitis B virus replication There was no significant difference between the positive rate of TTVDNA and the negative rate of TTVDNA (P> 0.05). ALT and TBIL in patients with chronic hepatitis B and chronic severe hepatitis B were significantly different between TTVDNA positive patients and TTVDNA negative patients (P <0.05), TTVDNA positive patients was significantly higher than TTVDNA negative patients Patient group. Conclusion: TTV infection exists in blood donors, and hepatitis T virus infection is more common in patients with TTV infection. The positive rate of TTVDNA in patients with chronic hepatitis B and chronic severe hepatitis B was significantly higher than that of blood donors and carriers of chronic hepatitis B virus, suggesting that the overlap infection of TTV may be one of the aggravating factors of HBV infection. TTV overlap infection HBV replication may not exist interaction.