通过降低培养基中铵态氮的浓度,使用谷酰胺作为无毒氮源,提高了小麦绿色苗的产量。在这样的培养基上,可以直接从小孢子发育成胚状体。这种胚状体在上述诱导培养基上发芽,通常可获得绿苗,因此,明显地减少了愈伤组织的形成。检验了在液体培养大麦花药中作为载体剂的聚蔗糖。用这种载体剂后,由于有反应的花药数和有结构发育的数目的增加而使胚状体的总数较高。当花药漂浮在聚蔗糖培养基的表面时,小孢子在花药里能够得到发育。在经过几次有丝分裂之后,花药壁破裂,原胚状体便被排出到培养基的表面上,发芽并继续发育。作者发现,在含聚蔗糖的液体培养基中用谷酰胺或天门冬酰胺代替胺态氮,对培养物的发育是有利的。作者用以上方法获得了6827个绿色植株,其绿苗与白苗的平均比值为1.8,每个花药的绿苗平均再生率为4.6个。 By reducing the concentration of ammonium nitrogen in the medium and using glutamine as a non-toxic nitrogen source, the yield of wheat green seedlings was increased. On such media, embryoids can develop directly from microspores. Such embryoid bodies germinate on the above induction medium, and usually green shoots are obtained, thereby remarkably reducing the callus formation. Ficoll was tested as a carrier in liquid cultured barley anthers. With this vector, the total number of embryoid bodies is higher due to the increased number of reacted anthers and the number of structurally developed. When the anthers float on the surface of the Ficoll medium, the microspores can develop in the anther. After several mitoses, the anther wall ruptures and the original embryoid bodies are expelled onto the surface of the medium, germinate and continue to develop. The authors found that the replacement of amine nitrogen with glutamine or asparagine in a liquid medium containing sucrose is advantageous for the development of the culture. The author obtained 6,827 green plants by the above method, the average ratio of green and white seedlings is 1.8, and the average regeneration rate of green plants per anther is 4.6.