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目的:分析不同采收途径及不同部位的白花丹中白花丹醌含量,寻找富集白花丹醌的药用部位和白花丹的最佳采收途径。方法:采用HPLC法测定不同采收途径白花丹和不同部位中白花丹醌的含量:色谱柱为Hypersil ODS(25cm×4.6mm,25μm),流动相为甲醇-水(65∶35),流速为1.0mL/min,检测波长为213nm,柱温30℃。结果:白花丹醌在0.01μg～0.32μg含量范围呈线性关系,Y=18211600X+10434.57758,r=0.99946,RSD=1.28%(n=6);新鲜的白花丹根部、茎部及叶中白花丹醌的含量分别为0.026%、0.006%和0.014%;干燥的白花丹根部、茎部及叶中白花丹醌的含量分别为0.324%、0.082%和0.174%;枯萎的白花丹茎部、叶及穗中白花丹醌的含量分别为0.002%、0.001%和0.003%。结论:干燥组白花丹中白花丹醌含量高于新鲜组和枯萎组,白花丹根部的白花丹醌含量最高,其次是穗,叶、茎。本法简便、准确,灵敏度高,重复性好,可用于控制白花丹药材的质量。
OBJECTIVE: To analyze the content of plumbagin in different ways and different parts of plumbagin, and to find out the best ways of harvesting the medicinal parts of plumbagin and plumbagin. Methods: The HPLC method was used to determine the content of plumbagin in different accessions. The chromatographic column was Hypersil ODS (25cm × 4.6mm, 25μm), the mobile phase was methanol-water (65:35), the flow rate was 1.0mL / min, detection wavelength of 213nm, column temperature 30 ℃. Results: Plumbagin was linear in the range of 0.01μg ~ 0.32μg, Y = 18211600X + 10434.57758, r = 0.99946, RSD = 1.28% (n = 6) The contents of quinone in the roots, stems and leaves of P. hupehensis were 0.324%, 0.082% and 0.174%, respectively. The contents of stem, leaf and Panax quinquefolium content were 0.002%, 0.001% and 0.003% respectively. Conclusion: The content of plumbagin in the dried group was higher than that in the fresh group and the wilt group. The content of plumbagin in the root of P. hupehensis was the highest, followed by the spike, leaf and stem. This method is simple, accurate, high sensitivity, reproducibility, and can be used to control the quality of Chinese medicine Huadu Dan.