论文部分内容阅读
目的 :用甘油复制兔急性肾小管坏死 (ATN)模型 ,观察当归、三七对ATN的保护作用 ,并初步探讨其机制。方法 :4 0只健康新西兰白色家兔 ,等分为四组 :当归组、三七组、对照组、正常组。当归、三七、对照三组均用 5 0 %甘油等渗盐水 15ml/kg注入兔双后肢皮下 ,复制ATN模型 ,当归组于注射甘油后即刻 ,第 3小时 ,第 6小时 ,肌注当归注射液 5 0mg/kg ,三七组于相同时点肌注三七皂甙 5 0mg/kg ,正常组则于兔双后肢皮下注射生理盐水 15ml/kg ,并于相同时点注射 5 %葡萄糖注射液。于实验第 2 4小时取血液、尿液和肾脏 ,检测血清尿素氮、肌酐、尿液总渗透压、尿总氨基酸、肾组织MDA、SOD、GSH -PX、ATP酶、Ca2 + 、肾组织病理形态学检查。结果 :各组与正常组比 ,肌酐、尿素氮明显升高 ,说明模型复制成功。当归、三七组无 1例死亡 ,对照组死亡率为 30 % ,血清肌酐、尿素氮、丙二醛、尿总氨基酸、滤过钠排泄分数和肾组织钙含量均明显低于对照组 (P <0 .0 1) ,而肾组织ATP酶、SOD、GSH -PX活性均高于对照组 (P <0 .0 1) ,病理形态学变化轻于对照组。以上指标 ,当归与三七组无显著差异 (P >0 .0 5 )。结论 :当归、三七对甘油所致的ATN有明显保护作用 ,二者效果无差异 ,其机制可能与二者均有改善微循环、血液流变学、抑
Objective : To replicate the rabbit acute tubular necrosis (ATN) model with glycerol, observe the protective effect of Angelica sinensis and Panax notoginseng on ATN, and preliminary explore its mechanism. Methods : Forty healthy New Zealand white rabbits were equally divided into four groups: Angelica group, Sanqi group, control group and normal group. Radix Angelicae Sinensis, Panax notoginseng and control group were infused with 50% glycerol isotonic saline 15ml/kg subcutaneously into the hind limbs of rabbits. The ATN model was reproduced. Immediately after the injection of glycerol in the Angelica group, at the 3rd hour and 6th hour, the intramuscular injection of Angelica Sinensis was injected. At 50 mg/kg, the Panax notoginseng group received intramuscular injection of Panax notoginseng 50 mg/kg at the same time point. In the normal group, normal saline was injected subcutaneously into the hind limbs of the rabbit at 15 ml/kg, and 5% glucose injection was injected at the same time. Blood, urine, and kidneys were taken at the 24th hour of the experiment to measure serum urea nitrogen, creatinine, total urine osmotic pressure, total urine amino acid, renal tissue MDA, SOD, GSH-PX, ATPase, Ca2+, renal histopathology. Morphological examination. Results: Compared with the normal group, creatinine and urea nitrogen were significantly increased in each group, indicating that the model was successfully reproduced. There was no death in the Angelica and Panax notoginseng group, and the mortality in the control group was 30%. Serum creatinine, urea nitrogen, malondialdehyde, total urinary amino acid, filtered sodium excretion, and renal calcium were all significantly lower than those in the control group (P). <0. 0 1), while the activities of ATPase, SOD and GSH-PX in kidney tissue were higher than those in the control group (P < 0.01), and the pathological changes were lighter than those in the control group. With the above indicators, there was no significant difference between Angelica and Panax notoginseng (P > 0.05). Conclusion: Angelica and Panax notoginseng have obvious protective effect on glycerol-induced ATN, and there is no difference between the two effects. The mechanism may be both improved microcirculation, hemorheology, and suppression.