【摘 要】
：
The chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus,which has infected millions of people in Africa,Asia,Americas,and Europe since it reemerged in India and Indian Ocean regions in 2005-2006.Starting in the middle of November 2016,CHIKV has
【机 构】
：
Chinese Academy of Sciences Key Laboratory of Special Pathogens and Biosafety, Center for Emerging I
论文部分内容阅读
The chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus,which has infected millions of people in Africa,Asia,Americas,and Europe since it reemerged in India and Indian Ocean regions in 2005-2006.Starting in the middle of November 2016,CHIKV has been widely spread,and more than 4,000 cases of infections in humans were confirmed in Pakistan.Here,we report the first isolation and characterization of CHIKV from the Pakistan outbreak.Eight CHIKV strains were newly isolated from human serum samples using a cell culture procedure.A full-length genome sequence and eight complete envelope (E1) sequences of CHIKV from Pakistan were obtained in this study.Alignment of the CHIKV E1 sequences revealed that the eight new CHIKV isolates were highly homogeneous,with only two nonsynonymous substitutions found at generally conserved sites (E99 and Q235).Based on the comparison of 342 E1 sequences,the two nonsynonymous mutations were located in well-recognized domains associated with viral functions such as the cell fusion and vector specificity,suggesting their potential functional importance.Phylogenetic analysis indicated that the CHIKV strains from Pakistan originated from CHIKV circulating in the Indian region.This study helps elucidate the epidemics of CHIKV in Pakistan and also provides a foundation for studies of evolution and expansion of CHIKV in South Asia.
其他文献
Rabies remains a public health threat that kills approximately 59,000 people worldwide each year,most of which are from the developing countries of Africa and Asia where dog rabies are endemic.Therefore,developing an affordable and efficacious vaccine is
We describe the first genome isolation of Middle East respiratory syndrome coronavirus (MERS-CoV) in Kenya.This fatal zoonotic pathogen was first described in the Kingdom of Saudi Arabia in 2012.Epidemiological and molecular evidence revealed zoonotic tra
Natural killer (NK) cell is a key component of innate immunity and plays an important role in host defense against virus infection by directly destroying infected cells.Influenza is a respiratory disease transmitted in the early phase of virus infection.E
The major difficulties of human papillomavirus (HPV) treatment are its persistence and recurrence.The HPV E7 oncoprotein-loaded dendritic cells have been evaluated as cellular vaccine in previous reports.Plasmacytoid dendritic cells (pDCs) play an essenti
Hepatitis C virus (HCV) infects approximately 180 million people worldwide.Significant progress has been made since the establishment of in vitro HCV infection models in cells.However,the replication of HCV is complex and not completely understood.Here,we
The relationship between the severity of dengue infection and allergy is still obscure.We conducted an electronic search across 12 databases for relevant articles reporting allergic symptoms,dengue infection,and dengue classification.These studies were ca