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目的:了解运脾滋肾汤对去卵巢大鼠骨丢失的影响及可能的作用机制,进而探讨PMOP发病机制以及为临床PMOP防治用药选择提供实验依据。方法:将成年雌性大鼠40只随机分为4组,①假手术(Sham)组②卵巢切除(OVX)组③卵巢切除+尼尔雌醇(OVX+E)组④卵巢切除+运脾滋肾汤(OVX+Y)组,分别对各组大鼠进行灌胃给药后处死全部大鼠,称取大鼠子宫湿重,测量子宫内膜厚度,测定血清钙、磷、碱性磷酸酶、性激素、骨钙素水平等代谢指标。结果:较OVX组相比,OVX+Y组大鼠BMD明显增加,血清钙、磷浓度亦血清中骨钙素浓度升高,碱性磷酸酶活性降低,差异均有显著性,而与OVX+E组相比则无明显差异,但大鼠子宫/体重指数较小,子宫内膜厚度较薄。结论:运脾滋肾汤可防治OVX所致骨丢失,其作用机制是通过对机体多方位、多环节的调节作用来实现的。
Objective: To understand the effect of Yunpi Zishen Decoction on bone loss in ovariectomized rats and its possible mechanism, and to explore the pathogenesis of PMOP and to provide experimental evidence for the prevention and treatment of clinical PMOP. Methods: Forty adult female rats were randomly divided into 4 groups: ① sham group ② ovariectomized (OVX) group ③ ovariectomy + nylestriol (OVX + E) group ④ ovariectomy + (OVX + Y) group. Rats in each group were given gavage, and all the rats were sacrificed. The wet weight of uterus was weighed, the thickness of uterus was measured, and the levels of serum calcium, phosphorus and alkaline phosphatase , Sex hormones, osteocalcin levels and other metabolic indicators. Results: Compared with OVX group, the BMD of OVX + Y group was significantly increased. Serum calcium and phosphorus concentrations also increased serum osteocalcin concentration and alkaline phosphatase activity, the difference was significant, but not with OVX + E group compared with no significant difference, but the rat uterus / body mass index smaller, thinner endometrial thickness. Conclusion: Yunpi Zishen Decoction can prevent and treat bone loss induced by OVX. Its mechanism is through the multi-orientation and multi-links regulation.