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纳米技术被广泛应用于医药领域,其能够显著提高药物的生物利用度及靶向递送能力。然而,纳米药物在体内环境中会吸附生物分子而影响其效能的发挥。已有的研究证明,血浆中的蛋白质吸附在纳米粒表面后会改变纳米粒的表面性质。纳米粒在细胞转运过程中,同样可能会吸附细胞中的某些蛋白。目前,研究者们并不知道这些蛋白是否会影响纳米粒在下层细胞中的摄取。为了简化研究,本研究将白蛋白包被的金纳米粒与Caco-2细胞裂解液共同孵育模拟纳米粒经上皮细胞转运过程;利用TEM观察纳米粒与细胞裂解液孵育前后的表面形态;使用SRB法评价孵育前后的纳米粒的细胞毒性;通过ICP-MS和CLSM实验测定金纳米粒在细胞中的摄取量和胞内分布情况。实验结果表明,细胞蛋白的吸附能够增加金纳米粒在下层细胞的粘附和细胞摄取。裂解液孵育的金纳米粒和对照组纳米粒都主要分布在细胞的溶酶体中,不同的是裂解液孵育的金纳米粒在线粒体有少量分布,而对照组金纳米粒在线粒体中几乎没有分布。
Nanotechnology is widely used in the field of medicine, which can significantly improve the bioavailability of drugs and the targeted delivery ability. However, nanomedicine can adsorb biomolecules in the environment of the body and affect its performance. Some studies have shown that the adsorption of plasma proteins on the surface of nanoparticles changes the surface properties of the nanoparticles. Nanoparticles in the cell transport process, the same may be the adsorption of certain proteins in the cell. At present, researchers do not know whether these proteins affect the uptake of nanoparticles in the underlying cells. In order to simplify the study, albumin-coated gold nanoparticles were co-incubated with Caco-2 cell lysate to simulate the transit of epithelial cells via nanoparticle. TEM was used to observe the surface morphology of the nanoparticles before and after incubation with cell lysate. Method to evaluate the cytotoxicity of nanoparticles before and after incubation. The uptake and intracellular distribution of gold nanoparticles in the cells were determined by ICP-MS and CLSM. The experimental results show that the adsorption of cellular proteins can increase the adhesion and cellular uptake of gold nanoparticles in the lower cells. Lysates incubated with gold nanoparticles and control nanoparticles were mainly distributed in the cell lysosomes, the difference is that the lysate incubated gold nanoparticles in a small amount of mitochondria distribution, while the control group of gold nanoparticles in the mitochondria almost no distributed.